Welcome, Guest ( Login )

InstantBlue - Protein Stain

InstantBlue - Protein Stain

Expedeon's InstantBlue is the only true single step Coomassie based gel stain available. Simple take the gel from it's cassette, put in stain and leave. No need for washing, microwaving or destaining. All bands will be visible within 15 Minutes. Gels can be left in InstantBlue indefinitely without any chance of background staining. InstantBlue is non-toxic and compatible with Mass Spectroscopy analysis.

Contents: 1 x 1 litre bottle of InstantBlue

Click for full description
Download product manual
Download protocol
Download MSDS's

 
Our PricePOA / Call
CodeISB1L
Kg1.2
 

InstantBlue
One Step gel
staining in 15 minutes or less.

 

InstantBlue is formulated ready for use. Gels can remain in the stain for weeks without concern. Only the proteins are stained, resulting in extremely well defined blue bands on a highly transparent background.

The reduction of background interference results in a better signal to noise ratio. Protein bands containing as low as 5 ng per band (BSA) can be detected.

The InstantBlue formulation is non-toxic and does not contain any methanol. Proteins stained using the InstantBlue stain are also compatible with mass spectrometry (MS) analysis.

Comparison with other stains

 





Benefits


  • Ultra-fast staining - providing results in 15 minutes or less.
  • Single step procedure - no need to wash, fix, microwave or destain. Just take the gel out of the cassette and place in a container with InstantBlue.
  • High sensitivity - approximately 5-25ng of protein detected per band.
  • Super-Low background staining - Improves overall gel resolution and sensitivity and no possibility of over staining means that you can leave your gel in the stain indefinitely and still be able to read it.
  • Quantitative - Same gel-to-gel performance ideal for quantifying protein by densitometry
  • Non-toxic - ideal for regular use and disposal.
  • Mass Spec Compatible1.

1 - Zhu, Z., Boobis, A.R., Edwards, R.J., Identification of estrogen-responsive proteins in MCF-7 human breast cancer cells using label-free quantitative proteomics, Proteomics 2008, 8, 1987-2005.



Product Information

Catalogue Number Description Storage Shelf Life
ISB1L InstantBlue, 1 Litre +4C 1 Year from receipt

Please review these other products:

 
RunBlue LDS Sample Buffer 10ml (4x Conc.)
RunBlue LDS Sample Buffer should be used to prepare all samples for runing on RunBlue  Precast Gels.  Specifically formulated for the RunBlue buffer system to provide the best running conditions and results.

Contents:  1 x Vial containing 10 ml LDS Sample Buffer (4x Conc.)
Our Price: POA / Call

click to see more

 
 
SDS 17 well, 10x10, SDS-PAGE Precast Gels
View

10 individually sealed, 17 well, 10x10 cm Gels.
Dimensions LxWxH (cm): 14 x 14 x 9
Weight (Kg): 0.9
Shipping: Ambient
Storage: +4 deg C

Click for full description
Download MSDS's
Download Product Manual

click to see more

 
 
17 well, 8x10, SDS-PAGE Precast Gels
View

10 individually sealed, 17 well, 8x10 cm Gels.
Dimensions LxWxH (cm): 14 x 14 x 9
Weight (Kg): 0.8
Shipping: Ambient
Storage: +4 deg C

Click for full description
Download MSDS's
Download Product Manual

 

click to see more

 
 
12 well, 8x10, SDS-PAGE Precast Gels
View


10 individually sealed, 12 well, 8x10 cm Gels.
Dimensions LxWxH (cm): 14 x 14 x 9
Weight (Kg): 0.8
Shipping: Ambient
Storage: +4 deg C

Click here for full description
Download MSDS's
Download Product Manual

 

click to see more

 
 
Runblue MIDI dual run & Blot unit

Expedeon's RunBlue MIDI dual run and Blot Unit for performing SDS-PAGE, acrylamide-nucleic acid separations and electro-blotting. This dual unit provides the capability of running or blotting two midi gels (10x15 cm) simultaneously. Units include 2 blotting cassettes, 2 foam pads, and a white plastic reservoir conversion plate to allow for single runs.

Download product manual

Our Price: POA / Call

click to see more

 
 
SDS 12 Well 10x10, SDS-PAGE Precast Gels
View
10 individually sealed, 12 well, 10x10 cm Gels.
Dimensions LxWxH (cm): 14 x 14 x 9
Weight (Kg): 0.9
Shipping: Ambient
Storage: +4 deg C

Click for full details below
Download MSDS's
Download Product Manual

click to see more

 
 
RunBlue Native Sample Buffer
View

All RunBlue Buffers, Reagents and Accessories have been specifically designed and formulated for use with the RunBlue gels and apparatus to provide optimum performance and results. Buffers are made using the highest quality chemicals and have different compositions to other manufacturers buffers.

Only use RunBlue formulated buffers with RunBlue gels.

Click for full description
Download protocol
Download Run Buffer MSDS's
Dwonload Sample Buffer MSDS's

click to see more

 
 
RunBlue Tris Glycine Blot Buffer
View

All RunBlue Buffers, Reagents and Accessories have been specifically designed and formulated for use with the RunBlue gels and apparatus to provide optimum performance and results. Buffers are made using the highest quality chemicals and have different compositions to other manufacturers buffers.

Only use RunBlue formulated buffers with RunBlue gels.

Click for full description

click to see more

 
 
Quad Cool (runs 4 gels) with 1 bufferdam & 4 blotting cassettes

Expedeon's RunBlue Dual Run & Blot system provides you with a gel tank with unsurpassed functionality. The core module hinges open to accept gel cassettes or buffer dam and then simply clips in place to provide a completely sealed cathode chamber. This allows RunBuffer to be added and samples to be loaded outside of the tank. In addition, Safety Cover removal could not be easier, with two protruding posts to push against, it can be slid off smoothly!

Click for full description
Download product manual

Our Price: POA / Call

click to see more

 
 
SDS 2 Well 10x10, SDS-PAGE Precast Gels
View

10 individually sealed, 2 well, 10x10 cm Gels.
Dimensions LxWxH (cm): 14 x 14 x 9
Weight (Kg): 0.9
Shipping: Ambient
Storage: +4 deg C

Click for full description
Download MSDS's
Download Product Manual

 

click to see more

 
 
SDS 1 Well 10x10, SDS-PAGE Precast Gels
View

10 individually sealed, 1 well, 10x10 cm Gels.
Dimensions LxWxH (cm): 14 x 14 x 9
Weight (Kg): 0.9
Shipping: Ambient
Storage: +4 deg C

Click for full description
Download MSDS's
Download Product Manual

 

click to see more

 
 
SDS 12+2 Well 10x15, SDS-PAGE Precast Gels
View 10 individually sealed, 12+2 well, 10x15 cm Gels.
Dimensions LxWxH (cm): 14 x 20 x 9
Weight (Kg): 1.4
Shipping: Ambient
Storage: +4 deg C

Click for full description
Download MSDS's
Download Product Manual

click to see more

 
 
SDS 20 Well 10x15, SDS-PAGE Precast Gels
View 10 individually sealed, 20 well, 10x15 cm Gels.
Dimensions LxWxH (cm): 14 x 20 x 9
Weight (Kg): 1.4
Shipping: Ambient
Storage: +4 deg C

Click for full description
Download MSDS's
Download Product Manual

click to see more

 
 
SDS 26 Well 10x15, SDS-PAGE Precast Gels
View 10 individually sealed, 26 well, 10x15 cm Gels.
Dimensions LxWxH (cm): 14 x 20 x 9
Weight (Kg): 1.4
Shipping: Ambient
Storage: +4 deg C

Click for full details below
Download MSDS's
Download Product Manual

click to see more