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InstantBlue - Protein Stain

InstantBlue - Protein Stain

Expedeon's InstantBlue is the only true single step Coomassie based gel stain available. Simple take the gel from it's cassette, put in stain and leave. No need for washing, microwaving or destaining. All bands will be visible within 15 Minutes. Gels can be left in InstantBlue indefinitely without any chance of background staining. InstantBlue is non-toxic and compatible with Mass Spectroscopy analysis.

Contents: 1 x 1 litre bottle of InstantBlue

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One Step gel
staining in 15 minutes or less.


InstantBlue is formulated ready for use. Gels can remain in the stain for weeks without concern. Only the proteins are stained, resulting in extremely well defined blue bands on a highly transparent background.

InstantBlue is formulated ready to use. Gels can remain in stain for weeks without concern. Only proteins are stained resulting in extremely well defined blue bands on a highly transparent background. The reduction of background interference results in a better signal to noise ratio. Protein bands containing as low as 5 ng per band (BSA) can be detected.

The InstantBlue formulation is non-toxic and does not contain any methanol. Proteins stained using the InstantBlue stain are also compatible with mass spectrometry (MS) analysis.

Comparison with other stains



  • Ultra-fast staining - providing results in 15 minutes or less.
  • Single step procedure - no need to wash, fix, microwave or destain. Just take the gel out of the cassette and place in a container with InstantBlue.
  • High sensitivity - approximately 5-25ng of protein detected per band.
  • Super-Low background staining - Improves overall gel resolution and sensitivity and no possibility of over staining means that you can leave your gel in the stain indefinitely and still be able to read it.
  • Quantitative - Same gel-to-gel performance ideal for quantifying protein by densitometry
  • Non-toxic - ideal for regular use and disposal.
  • Mass Spec Compatible1.

1 - Zhu, Z., Boobis, A.R., Edwards, R.J., Identification of estrogen-responsive proteins in MCF-7 human breast cancer cells using label-free quantitative proteomics, Proteomics 2008, 8, 1987-2005.

Product Information

Catalogue Number Description Storage Shelf Life
ISB1L InstantBlue, 1 Litre +4C 1 Year from receipt